منابع مشابه
Semiquantitation of bacteria in sputum gram stains.
In many clinical laboratories, bacteria seen in Gram-stained sputum smears are reported semiquantitatively, using a three- or four-category scale consisting of ratings such as numerous, moderate, rare, and none seen. The consistency with which these categories are assigned was evaluated by repeatedly presenting coded smears to seven experienced microbiology technologists. Technologists rated th...
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We demonstrate the feasibility of using qPCR on DNA extracted from vaginal Gram stain slides to estimate the presence and relative abundance of specific bacterial pathogens. We first tested Gram stained slides spiked with a mix of 10(8) cfu/ml of Escherichia coli and 10(5) cfu/ml of Lactobacillus acidophilus. Primers were designed for amplification of total and species-specific bacterial DNA ba...
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Chemical studies have been carried out on the interaction of DNA with uranyl salts. The effect of variations in pH, salt concentration, and structural integrity of the DNA on the stoichiometry of the salt-substrate complex have been investigated. At pH 3.5 DNA interacts with uranyl ions in low concentration yielding a substrate metal ion complex with a UO(2) (++)/P mole ratio of about (1/2) and...
متن کاملClinical and laboratory analyses of cytospin-prepared Gram stains for recovery and diagnosis of bacteria from sterile body fluids.
The smear of a clinical specimen provides essential laboratory information that is used to make therapeutic decisions. For this study, smears were made by centrifugation in a Beckman Microfuge 11 (Beckman Instruments, Palo Alto, Calif.) and in parallel by using a Cytospin 2 apparatus (Shandon Inc., Pittsburgh, Pa.). Of 350 consecutive body fluid specimens examined, 50 (14.0%) grew bacteria. Bot...
متن کاملStains Other Than Blood-Stains as Medico-Legal Evidence
On one point an important difference of opinion may exist between medico-legal workers: should a positive chemical test, in the absence of finding spermatozoa under the microscope, be accepted a& evidence of semen ? Some are in favour of doing so (Bagchi, 1937), others against (Owens, 1935; Thomas, 1937; Kamath, 1938; Glaister, 1938; McNally, 1939; Modi, 1940; Rhodes, 1940) and yet others appea...
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ژورنال
عنوان ژورنال: Clinical Infectious Diseases
سال: 2010
ISSN: 1058-4838,1537-6591
DOI: 10.1093/cid/ciq131